Antibodies

Our laboratory developed a large series of monoclonal antibodies to a number of cytoskeletal  proteins. These antibodies  are shown in the tables and are available to other researchers directly on request. Some of the antibodies are available commercially through various companies, including Abcam, Abnova, Biolegend, Exbio, GeneTex, Novus Biologicals, Pierce, Santa Cruz Biotechnology, Sigma-Aldrich

Antibodies to tubulins

Specificity Epitope Clone Class References
α-tubulin, N-terminal structural domain α(65-79) TU-01 IgG1 1, 2, 3, 4, 5, 20, 24, 25, 26, 27, 28, 29, 31, 32, 33, 36, 37, 42, 46
α-tubulin, N-terminal structural domain   TU-02 IgM 3, 6
α-tubulin, N-terminal structural domain   TU-03 IgM 3, 6
α-tubulin, N-terminal structural domain   TU-05 IgM 7
α-tubulin, N-terminal structural domain   TU-09 IgM 3, 7, 8
α-tubulin, C-terminal structural domain   TU-04 IgM 3, 5, 8
α-tubulin, C-terminal structural domain α(406-410) TU-07 IgM 3, 8, 40
α-tubulin   TU-16 IgM 9, 21, 22, 23, 33
β-tubulin, N-terminal structural domain β(81-95) TU-06 IgM 3, 4, 5, 12, 20, 23, 26, 28, 29, 35, 37
β-tubulin, N-terminal structural domain   TU-13 IgM 7,12
β-tubulin, C-terminal structural domain   TU-11 IgM 3, 4
β-tubulin, C-terminal structural domain β(426-435) TU-12 IgM 8, 13, 27, 29, 35
β-tubulin, C-terminal structural domain β(436-445) TU-14 IgM 3, 4, 8, 35
β-tubulin, class III specific (ESESQGPK) β(441-448) TU-20 IgG1 14, 15, 25, 30 , 31, 45
αβ-tubulin dimer   TU-08 IgM 9
αβ-tubulin dimer   TU-10 IgM 10
αβ-tubulin dimer   TU-18 IgM 11
γ-tubulin (EYHAATRPDYISWGTQ) γ(439-444) TU-30 IgG1 16, 18, 19, 21, 24, 30, 32, 34, 37, 38, 39, 41, 43, 44, 45

γ-tubulin(EYHAATRPDYISWGTQ)

 γ(441-446) TU-31 IgG2b 16, 17, 18, 21, 22, 23, 24, 25, 27, 28, 30, 32, 34, 37, 41, 42, 43, 45,
γ-tubulin (EYHAATRPDYISWGTQ) γ(441-446) TU-32 IgG1 16, 17, 18, 21, 22, 24, 26, 27, 28, 30, 34, 37, 38, 39, 42, 43, 45

 

References
1. Viklický et al., Cell Biol. Int. Rep. 6: 725-731, 1982.  pdf
2. Grimm et al., Biochim. Biophys. Acta 914: 83-88, 1987.
3. Dráber et al., J. Cell Sci. 92: 519-528, 1989. pdf
4. Linhartová et al., Biochem. J. 288: 919-924, 1992. pdf
5. Smertenko et al., Planta 201: 349-358, 1997. pdf
6. Dráber et al., Eur. J. Cell Biol. 41: 82-88, 1986. pdf
7. Dráber et al., Histochemistry 89: 485-492, 1988. pdf
8. Smertenko et al., Eur. J. Cell Biol. 72: 104-112, 1997. pdf
9. Dráberová and Dráber , Fol. Biol. (Praha) 44: 35-36, 1998. pdf
10. Korenbaum et al., Protoplasma 154: 91-97, 1990
11. Dráberová et al., Histochem. Cell Biol. 109: 231-239, 1998. pdf
12. Dráber et al., FEBS Lett. 262: 209-211, 1990. pdf
13. Dráber et al., Histochemistry 95: 519-524, 1991. pdf
14. Dráberová et al., Histochem. Cell Biol. 109: 231-239, 1998. pdf
15. Zíková et al., FEBS Lett. 473: 19-23, 2000. pdf
16. Nováková et al., Cell Motil. Cytoskeleton 33: 38-51, 1996. pdf
17. Dráberová et al., Int. Immunol. 11: 1829-1839, 1999. pdf
18. Binarová et al., Plant Cell 12 : 433-442, 2000. pdf
19. Nohýnková et al., Eur. J. Cell Biol. 78: 438-445, 2000. pdf
20. Kratzerová et al., Exp. Cell Res. 266: 270-278, 2001. pdf
21. Linhartová et al., Develop. Dynamics 223: 229-240, 2002. pdf
22. Sulimenko et al., Biochem. J. 365: 889-895, 2002. pdf
23. Dryková et al., Plant Cell 15: 15: 465-480, 2003. pdf
24. Libusová et al., Exp. Cell Res. 295: 375-386, 2004. pdf
25. Kukharskyy et al., Exp. Cell Res. 298: 218-228, 2004. pdf
26. Libusová et al., Protoplasma 225: 157-167, 2005. pdf
27. Katsetos et al., J. Neuropathol. Exp. Neurol. 65: 465-477, 2006. pdf
28. Sulimenko et al., J. Immunol. 176: 7243-7253, 2006. pdf
29. Pěknicová et al., Fertil. Steril. 88: 1120-1128, 2007. pdf
30. Katsetos et al., Neurochem. Res. 32: 1387-1398, 2007. pdf
31. Dráberová et al., J. Neuropathol. Exp. Neurol. 67: 341-354, 2008. pdf
32. Macurek et al., Biochem J. 416: 421-430, 2008. pdf
33. Blume et al., Planta 229: 143-150, 2008. pdf
34. Caracciolo et al., J. Cell Physiol. 223: 519-529, 2010. pdf
35. Blume et al., BMC Plant Biol. 10: e29, 2010. pdf
36. Dráberová et al., J. Neuropathol. Exp. Neurol. 70: 811-826, 2011. pdf
37. Hořejší et al., J. Cell Physiol. 227: 367-382, 2012. pdf
38. Vinopal et al., PLoS ONE 7: e29919, 2012. pdf
39. Maounis et al., Histol. Histopathol. 27: 1183-1194, 2012. pdf
40. Dráberová et al., J. Immunol. Methods 395: 63-70, 2013. pdf
41. Sulimenko et al., J. Immunol. 194: 4099-4111, 2015. pdf
42. Dráberová et al., J. Neuropathol. Exp. Neurol. 74: 723-742, 2015. pdf
43. Černohorská et al., BBA Mol. Cell Res. 1863: 1282-1297, 2016. pdf
44. Sulimenko et al., Protoplasma 254: 1187-1199, 2017. pdf
45. Dráberová et al.,  FASEB J.  31: 1828-1848, 2017. pdf
46. Ghaly et al., Can. J. Chem. 95: 649-655, 2017. pdf

 

Antibodies to tubulin-associated proteins

Specificity Epitope Clone Class References
MAP2ab   MT-01 IgG1 1, 6
MAP2ab   MT-02 IgG1 1 ,6
MAP2ab   MT-03 IgG2b 2
MAP2ab   MT-04 IgG1 2
MAP2ab 905-925 MT-07 IgG1 in preparation
MAP2ab 1375-1395 MT-08 IgG1 in preparation
Kinesin, heavy chain, stalk domain   KN-01 IgM 3, 5
Kinesin, heavy chain, stalk domain   KN-02 IgM 4, 5
Kinesin, heavy chain   KN-03 IgM 4
γ-tubulin complex protein 2 (GCP2)   GCP2-01 IgG2b 7, 8, 9,10
γ-tubulin complex protein 2 (GCP2) 64-75 GCP2-02 IgG1 7, 8,10  
TPX2 622-662 TPX2-01 IgG1 in preparation

 

References
1. Riederer et al., J. Histochem. Cytochem. 43: 1269-1284, 1995. pdf
2. Zíková et al., Fol. Biol. (Praha) 46: 87-88, 2000. pdf
3. Dráberová et al., Fol. Biol. (Praha) 48:77-79, 2002. pdf
4. Macurek et al., Hybridoma and Hybridomics 21: 457-462, 2002. pdf
5. Malcová-Janatová et al., Fol. Microbiol. 49: 665-670, 2004. pdf
6. Dráberová et al., J. Neuropathol. Exp. Neurol. 67: 341-354, 2008. pdf
7. Hořejší et al., J. Cell Physiol. 227: 367-382, 2012. pdf
8. Vinopal et al., PLoS ONE 7: e29919, 2012. pdf
9. Sulimenko et al., J. Immunol. 194: 4099-4111, 2015. pdf
10. Dráberová et al., J. Neuropathol. Exp. Neurol. 74: 723-742, 2015. pdf

 

Antibodies to intermediate filament proteins

Specificity Epitope Clone Class References
Vimentin   VI-01 IgM 1, 2, 3, 6, 9, 10, 11, 13
Vimentin   VI-10 IgM 4,11
GFAP   GF-01 IgG1 2, 7, 11
GFAP   GF-02 IgM 7
NF-H, phosphorylated epitope   NF-01 IgG1 3,11, 13
NF-H   NF-02 IgG1 8
NF-H, phosphorylated epitope   NF-03 IgM 8
NF-H, phosphorylated epitope   NF-04 IgG1 8
NF-H   NF-05 IgM 8
NF-H, phosphorylated epitope   NF-07 IgG1 8
NF-M   NF-08 IgG1 8
NF-M, unphosphorylated epitope   NF-09 IgG2a 5,11, 12
NF-M   NF-10 IgM 8

 

References
1. Dráberová et al., Fol. biol. (Praha) 32: 295-303, 1986. pdf
2. Lukáš et al., Histochemical J. 21: 693-702, 1989. pdf
3. Lukáš et al., Histochemistry 100: 495-502, 1993. pdf
4. Dráberová et al., Fol. Biol. (Praha) 45: 35-36, 1998. pdf
5. Dráberová et al., Fol. Biol. (Praha) 45: 163-165, 1999. pdf
6. Kratzerová et al., Exp. Cell Res. 266: 270-278, 2001. pdf
7. Porchet et al., Proteomics 3: 1476-1485, 2003. pdf
8. Porchet et al., NeuroReport 14: 929-933, 2003. pdf
9. Libusová et al., Exp. Cell Res. 295: 375-386, 2004. pdf
10. Sulimenko et al., J. Immunol. 176: 7243-7253, 2006. pdf
11. Dráberová et al., J. Neuropathol. Exp. Neurol. 67: 341-354, 2008. pdf
12. Dráberová et al.,  FASEB J.  31: 1828-1848, 2017. pdf
13. Lukáš et al., Fol. Biol. (Praha) 37: 207-212, 1991. pdf

 

Antibodies to other proteins

Specificity Epitope Clone Class References
Transferrin   HTF-14 IgG1 1
Horseradish peroxidase   HP-03 IgG1 2, 3
Beta-galactosidase   BG-02 IgG1 4, 5
Clathrin, heavy chain   BF-06 IgM 6
RPSA 117-132 RP-01 IgG2a in preparation
RPSA 117-132 RP-02 IgG2a in preparation

 

References
1. Bártek et al., Fol. Biol. (Praha) 30: 137-140, 1984. pdf
2. Viklický et al., Fol. Biol. (Praha) 30: 377-381, 1984. pdf
3. Dráber et al., Acta histochem. 81: 77-83, 1987. pdf
4. Slavíčková et al., Fol. Biol. (Praha) 38: 350-357, 1992. pdf
5. Dráber et al., Hybridoma 11: 385-390, 1992. pdf
6. Macurek et al., Fol. Biol. (Praha) 49: 238-240, 2003. pdf

 

Links to distributors of our antibodies

PDF download

All antibodies with references in one pdf.

Antibodies-3.pdf

Antibodies
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